Mutational analysis and molecular modeling of the binding of Staphylococcus aureus enterotoxin C2 to a murine T cell receptor Vbeta10 chain.

@article{Rssle2002MutationalAA,
  title={Mutational analysis and molecular modeling of the binding of Staphylococcus aureus enterotoxin C2 to a murine T cell receptor Vbeta10 chain.},
  author={Shaila C. R{\"o}ssle and Paulo Mascarello Bisch and Yu-chun Lone and J. -P. Abastado and P. Kourilsky and Maria Bellio},
  journal={European journal of immunology},
  year={2002},
  volume={32 8},
  pages={
          2172-8
        }
}
We investigated the role of the beta chain HV4 region in the binding of a Vbeta10 T cell receptor to superantigen S. aureus enterotoxin C2 (SEC2)/MHC class II complexes. Residues 6971 of the Cw3/1.1 TCR Vbeta10 chain, derived from an H-2K(d)-restricted cytotoxic clone, were individually changed to alanine using site-directed mutagenesis, and mutated TCR beta chains were transfected along with the wild-type TCR alpha chain into a TCR alpha(-)beta(-) T hybridoma. SEC2/MHC recognition was measured… CONTINUE READING
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