Mutation to rifampicin resistance at the beginning of the RNA polymerase β subunit gene in Escherichia coli

@article{Lisitsyn1984MutationTR,
  title={Mutation to rifampicin resistance at the beginning of the RNA polymerase β subunit gene in Escherichia coli},
  author={Nikolai A. Lisitsyn and E. D. Sverdlov and Elena P. Moiseyeva and Olga N Danilevskaya and Vadim G. Nikiforov},
  journal={Molecular and General Genetics MGG},
  year={1984},
  volume={196},
  pages={173-174}
}
The unusual recombinant plasmid pRC19 carrying the N-terminal fragment of the Escherichia coli RNA polymerase rpoB gene was found to specify high level rifampicin resistance of E. coli cells. Sequence analysis of this plasmid revealed one substitution only: transversion G→T, leading to amino acid substitution Val146→Phe. This mutational change marks the second domain of the β subunit involved in rifampicin binding.