Mutation screening of the TP53 gene by temporal temperature gradient gel electrophoresis.

@article{Srlie2005MutationSO,
  title={Mutation screening of the TP53 gene by temporal temperature gradient gel electrophoresis.},
  author={Th{\'e}rese S\orlie and Hilde Johnsen and Phuong Vu and Guro E Lind and Ragnhild A. Lothe and Anne-Lise B\orresen-Dale},
  journal={Methods in molecular biology},
  year={2005},
  volume={291},
  pages={207-16}
}
A protocol for detection of mutations in the TP53 gene using temporal temperature gradient gel electrophoresis (TTGE) is described. TTGE is a mutation detection technique that separates DNA fragments differing by single base pairs according to their melting properties in a denaturing gel. It is based on constant denaturing conditions in the gel combined with a temperature gradient during the electrophoretic run. This method combines some of the advantages of the related techniques denaturing… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

Similar Papers

Loading similar papers…