Mutation of the highly conserved Arg165 and Glu168 residues of human Gsα disrupts the αD–αE loop and enhances basal GDP/GTP exchange rate

@article{Hinrichs2004MutationOT,
  title={Mutation of the highly conserved Arg165 and Glu168 residues of human Gs$\alpha$ disrupts the $\alpha$D–$\alpha$E loop and enhances basal GDP/GTP exchange rate},
  author={Mar{\'i}a Victoria Hinrichs and Mart{\'i}n A. Montecino and Marta Bunster and Juan Olate},
  journal={Journal of Cellular Biochemistry},
  year={2004},
  volume={93}
}
G protein signalling regulates a wide range of cellular processes such as motility, differentiation, secretion, neurotransmission, and cell division. G proteins consist of three subunits organized as a Gα monomer associated with a Gβγ heterodimer. Structural studies have shown that Gα subunits are constituted by two domains: a Ras‐like domain, also called the GTPase domain (GTPaseD), and an helical domain (HD), which is unique to heterotrimeric G‐proteins. The HD display significantly higher… 
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