Mutation of the fourth cytoplasmic loop of rhodopsin affects binding of transducin and peptides derived from the carboxyl-terminal sequences of transducin alpha and gamma subunits.

@article{Ernst2000MutationOT,
  title={Mutation of the fourth cytoplasmic loop of rhodopsin affects binding of transducin and peptides derived from the carboxyl-terminal sequences of transducin alpha and gamma subunits.},
  author={Oliver P Ernst and Clinton K. Meyer and Ethan P Marin and Peter Henklein and Wang Yang Fu and Thomas P. Sakmar and Klaus Peter Hofmann},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 3},
  pages={1937-43}
}
The role of the putative fourth cytoplasmic loop of rhodopsin in the binding and catalytic activation of the heterotrimeric G protein, transducin (G(t)), is not well defined. We developed a novel assay to measure the ability of G(t), or G(t)-derived peptides, to inhibit the photoregeneration of rhodopsin from its active metarhodopsin II state. We show that a peptide corresponding to residues 340-350 of the alpha subunit of G(t), or a cysteinyl-thioetherfarnesyl peptide corresponding to residues… CONTINUE READING

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