Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans

  title={Mutation of a gene that encodes a kinesin-like protein blocks nuclear division in A. nidulans},
  author={A P Enos and N. Ronald Morris},

Mitotic regulation in Aspergillus nidulans.

Results indicate that the mitotic block caused by inactivation of bimE requires activation of the p34cdc2/MPF kinase for chromatin condensation and spindle polymerization to occur.

Suppression of the bimC4 mitotic spindle defect by deletion of klpA, a gene encoding a KAR3-related kinesin-like protein in Aspergillus nidulans

It is hypothesized that the loss of KLPA function redresses unbalanced forces within the spindle caused by mutation in bimC, and that the KLPA and BIMC kinesin-like proteins may play opposing roles in spindle function.

The pot1+ homologue in Aspergillus nidulans is required for ordering mitotic events

It is concluded that nimU is constitutively required for orderly mitotic progression under normal growth conditions and also required for the conditional mitotic spindle checkpoint response.

bimA encodes a member of the tetratricopeptide repeat family of proteins and is required for the completion of mitosis in Aspergillus nidulans.

Molecular disruption of the wild-type bimA gene is recessive in the heterokaryon and causes a metaphase block, demonstrating that bIMA is an essential gene for mitosis.

A structure-function analysis of NOD, a kinesin-like protein from Drosopbila melanogaster

Four of the mutations are missense and affect highly conserved domains of the kinesin-like portion of the predicted protein, and thus demonstrate that the sequence conservation is biologically relevant, and it is suggested that this part of the protein may also be essential for wild-type function.

A dynamin-like protein encoded by the yeast sporulation gene SP015

The SPO15 gene product expressed in Escherichia coli can be affinity-purified with microtubules and encodes a protein that is likely to be involved in a microtubule-dependent process required for the timely separation of spindle-pole bodies in meiosis.

Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly

Findings suggest that two Saccharomyces cerevisiae genes, CIN8 and KIP1, interact with spindle microtubules to produce an outwardly directed force acting upon the poles.

Gamma-tubulin and the C-terminal motor domain kinesin-like protein, KLPA, function in the establishment of spindle bipolarity in Aspergillus nidulans.

It is indicated, surprisingly, that gamma-tubulin and the klpA kinesin have overlapping roles in the establishment of spindle bipolarity.

A screen for dynein synthetic lethals in Aspergillus nidulans identifies spindle assembly checkpoint genes and other genes involved in mitosis.

Genetic interaction between dynein and spindle assembly checkpoint genes, as well as other mitotic genes, indicates that A. nidulans dyne in plays a role in mitosis.



Regulation of the mRNA levels of nimA, a gene required for the G2-M transition in Aspergillus nidulans

It is demonstrated not only that nimA is required for entry into mitosis, but because the transcript is normally expressed cyclically and is under tight cell cycle control, they suggest that nIMA may play a regulatory role in the initiation of mitosis.

A mutation in aspergillus nidulans that blocks the transition from interphase to prophase

From data, it is concluded that at restrictive temperature nimA5 blocks the nuclear division cycle at a point immediately preceding the initiation of chromosomal condensation and mitotic microtubule assembly, and upon shifting to permissive control over the initiation in vivo through a simple temperature shift and, consequently, nIMA5 should be a powerful tool for studying these processes.

Mitotic mutants of Aspergillus nidulans.

Forty-five temperature-sensitive mutants of Aspergillus nidulans which are defective in nuclear division, septation or distribution of nuclei along the mycelium have been isolated, and most have been

S-phase, G2, and nuclear division mutants of Aspergillus nidulans

Twenty-two temperature-sensitive cell cycle mutants of the fungus Aspergillus nidulans, which block in interphase at restrictive temperature, were analyzed by the reciprocal shift method to determine whether these mutations were blocked at the G1, S, or G2 phase of the cell cycle.