Mutation of Pro-258 in transmembrane domain 6 constitutively activates the G protein-coupled alpha-factor receptor.

@article{Konopka1996MutationOP,
  title={Mutation of Pro-258 in transmembrane domain 6 constitutively activates the G protein-coupled alpha-factor receptor.},
  author={James B Konopka and Steluta Mariana Margarit and Peter Dube},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 13},
  pages={6764-9}
}
The alpha-factor pheromone receptor stimulates MATa yeast cells to undergo conjugation. The receptor contains seven transmembrane domains that function in ligand binding and in transducing a signal to the cytoplasmic receptor sequences to mediate G protein activation. A genetic screen was used to isolate receptor mutations that constitutively signal in the absence of alpha-factor. The Pro-258-->Leu (P258L) mutation caused constitutive receptor signaling that was equivalent to about 45% of the… CONTINUE READING