Mutation of Conserved Histidines Alters Tertiary Structure and Nanomechanics of Consensus Ankyrin Repeats*

@inproceedings{Lee2012MutationOC,
  title={Mutation of Conserved Histidines Alters Tertiary Structure and Nanomechanics of Consensus Ankyrin Repeats*},
  author={Whasil Lee and Johan Str{\"u}mpfer and Vann Bennett and Klaus Schulten and Piotr E. Marszalek},
  booktitle={The Journal of biological chemistry},
  year={2012}
}
The conserved TPLH tetrapeptide motif of ankyrin repeats (ARs) plays an important role in stabilizing AR proteins, and histidine (TPLH)-to-arginine (TPLR) mutations in this motif have been associated with a hereditary human anemia, spherocytosis. Here, we used a combination of atomic force microscopy-based single-molecule force spectroscopy and molecular dynamics simulations to examine the mechanical effects of His → Arg substitutions in TPLH motifs in a model AR protein, NI6C. Our molecular… CONTINUE READING

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Mutation Effects on Ankyrin Repeat Mechanics 19120 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME

  • H. Lu, B. Isralewitz, A. Krammer, V. Vogel, K. Schulten
  • JUNE
  • 2012
1 Excerpt

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