Mutation detection in rice waxy mutants by PCR-RF-SSCP

@article{Sato2003MutationDI,
  title={Mutation detection in rice waxy mutants by PCR-RF-SSCP},
  author={Yo-ichiro Sato and Takeshi Nishio},
  journal={Theoretical and Applied Genetics},
  year={2003},
  volume={107},
  pages={560-567}
}
PCR-RF-SSCP (PRS), which combines cleaved amplified polymorphic sequence (CAPS) and single-strand conformation polymorphism (SSCP), is expected to be a useful technique for DNA polymorphism analysis. We evaluated the ability of PRS to detect single nucleotide polymorphism (SNP) using the Waxy gene, Wx, of rice, and subsequently were able to identify point mutations in wx mutant lines. The approximately 6-kb Wx gene was divided into five regions for PCR amplification. Two regions, in which most… CONTINUE READING
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