Mutation K448E in the external loop 5 of rat GABA transporter rGAT1 induces pH sensitivity and alters substrate interactions.

@article{Forlani2001MutationKI,
  title={Mutation K448E in the external loop 5 of rat GABA transporter rGAT1 induces pH sensitivity and alters substrate interactions.},
  author={Greta Forlani and Elena Bossi and R Ghirardelli and Stefano Giovannardi and Francesca Binda and Lorena Bonadiman and L Ielmini and Ant{\'o}nio Peres},
  journal={The Journal of physiology},
  year={2001},
  volume={536 Pt 2},
  pages={479-94}
}
1. The effect of the mutation K448E in the rat GABA transporter rGAT1 was studied using heterologous expression in Xenopus oocytes and voltage clamp. 2. At neutral pH, the transport-associated current vs. voltage (I-V) relationship of the mutated transporter was different from wild-type, and the pre-steady-state currents were shifted towards more positive… CONTINUE READING