Mutant rat trypsin selectively cleaves tyrosyl peptide bonds.

@article{Pl2004MutantRT,
  title={Mutant rat trypsin selectively cleaves tyrosyl peptide bonds.},
  author={G{\'a}bor P{\'a}l and Andr{\'a}s Patthy and J{\'o}zsef Antal and L{\'a}szl{\'o} Gr{\'a}f},
  journal={Analytical biochemistry},
  year={2004},
  volume={326 2},
  pages={
          190-9
        }
}
A double mutant of rat trypsinogen (Asp189Ser, DeltaAsp223) was constructed by site-directed mutagenesis. The recombinant protein was produced in Escherichia coli under the control of a periplasmic expression vector. The purified and enterokinase-activated enzyme was characterized by synthetic fluorogenic tetrapeptide and natural polypeptide substrates and by a recently developed method. In case of this latter method the specificity profile of the enzyme was examined by simultaneous digestion… CONTINUE READING

Figures, Tables, and Topics from this paper.

References

Publications referenced by this paper.
SHOWING 1-10 OF 37 REFERENCES

Gy

L. Graf, A. Jancso, L. Szilagyi
  • Hegyi, K. Pinter, G. Naray- Szabo, J. Hepp, K. Medzihradszky, W.J. Rutter, Electrostatic complementarity within the substrate-binding pocket of trypsin, Proc. Natl. Acad. Sci. USA 85
  • 1988
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

U. K. LAEMMLI
  • Nature
  • 1970
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

Chymotrypsin

L. Graf, L. Szilagyi, I. Venekei
  • in: A.J. Barrett, F. Woessner, N. Rawlings (Eds.), Handbook of Proteolytic Enzymes, Academic Press, London
  • 1998
VIEW 1 EXCERPT

Structural basis of substrate specificity in the serine proteases.

  • Protein science : a publication of the Protein Society
  • 1995
VIEW 2 EXCERPTS