Mutant huntingtin impairs post-Golgi trafficking to lysosomes by delocalizing optineurin/Rab8 complex from the Golgi apparatus.

@article{Toro2009MutantHI,
  title={Mutant huntingtin impairs post-Golgi trafficking to lysosomes by delocalizing optineurin/Rab8 complex from the Golgi apparatus.},
  author={Daniel del Toro and Jordi Alberch and Francisco L{\'a}zaro-Di{\'e}guez and Raquel Mart{\'i}n-Ib{\'a}{\~n}ez and Xavier Xifr{\'o} and Gustavo Egea and Josep M Canals},
  journal={Molecular biology of the cell},
  year={2009},
  volume={20 5},
  pages={
          1478-92
        }
}
Huntingtin regulates post-Golgi trafficking of secreted proteins. Here, we studied the mechanism by which mutant huntingtin impairs this process. Colocalization studies and Western blot analysis of isolated Golgi membranes showed a reduction of huntingtin in the Golgi apparatus of cells expressing mutant huntingtin. These findings correlated with a decrease in the levels of optineurin and Rab8 in the Golgi apparatus that can be reverted by overexpression of full-length wild-type huntingtin. In… CONTINUE READING