Mutant Escherichia coli penicillin acylase with enhanced stability at alkaline pH.

@article{Rio1995MutantEC,
  title={Mutant Escherichia coli penicillin acylase with enhanced stability at alkaline pH.},
  author={Gabriel del Rio and Myriam E. Rodriguez and Mar{\'i}a Elena Mungu{\'i}a and A L{\'o}pez-Mungu{\'i} and Xavier Sober{\'o}n},
  journal={Biotechnology and bioengineering},
  year={1995},
  volume={48 2},
  pages={141-8}
}
Increased stability at alkaline pH should be a valuable attribute for the utilization of penicillin acylase in bioreactors employed to convert penicillins into 6-aminopenicillanic acid, a precursor of semisynthetic penicillins. In these systems, base is added for pH control, which results in local alkaline conditions that promote enzyme inactivation… CONTINUE READING