Mutagenicity of urine from rats after administration of 2,4-diaminoanisole: the effect of microsomal enzyme inducers.

  title={Mutagenicity of urine from rats after administration of 2,4-diaminoanisole: the effect of microsomal enzyme inducers.},
  author={T. Reddy and T. Benjamin and P. H. Grantham and E. Weisburger and S. Thorgeirsson},
  journal={Mutation research},
  volume={79 4},
Ring 14C-labelled 2,4-diaminoanisole disulfate was administered to rats pretreated with the microsomal inducers phenobarbital (PB), beta-naphthoflavone (BNF), 3-methylcholanthrene (MC) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The 24-h urine from rats pretreated with PB showed a 2-fold increase in revertant rate over the corresponding control as measured by the Ames Salmonella test system. Pretreatment of rats with BNF, MC or TCDD decreased the mutagenicity of urine by about 70% when an… Expand
Influence of disulfiram on mutagenicity of the urinary metabolites of 2-acetylaminofluorene and N-hydroxy-2-acetylaminofluorene in the Salmonella test system.
Results indicate that DS increased the excretion of glucuronide metabolites of the carcinogens, which was increased appreciably in the presence of S9 and beta-glucuronidase. Expand
Methods for measuring mutagenicity in urine of rats dosed with [14C]di(2-ethylhexyl)phthalate.
The direct urine plating procedure represents a viable alternative to the above concentration procedures for this phthalate ester and the effects of L-histidine and the beta-glucuronidase/aryl sulfatase preparation on the background reversion frequencies of the Ames tester strains is discussed. Expand
Environmental fate, (bio)transformation, and toxicology of 2,4-dinitroanisole (DNAN) in soils and wastewater sludge
As the use of the insensitive munition compound 2,4-dinitroanisole (DNAN) increases, releases to the environment may pose a threat to local ecosystems. Little is known about the environmental fate ofExpand
Microbial toxicity of the insensitive munitions compound, 2,4-dinitroanisole (DNAN), and its aromatic amine metabolites.
Results indicate that reductive biotransformation could reduce the inhibitory potential of DNAN. Expand
Mutagens in urine of carbon electrode workers
It is suggested that application of the Salmonella/microsome test to work environments could offer useful and suitable tool for evaluating the health hazards due to mutagenic/carcinogenic substances from occupational exposure. Expand
Relationships between structure and mutagenic activity of environmental chemicals.
  • M. Shahin
  • Chemistry, Medicine
  • Mutation research
  • 1987
This review analyzes relationships between chemical structure and biological activity for several series of compounds. Its focus is on mutagenicity and carcinogenicity and the predictability of theseExpand
The mutagenicity and carcinogenicity of hair dyes
  • D. Kirkland
  • Chemistry, Medicine
  • International journal of cosmetic science
  • 1983
Some hair dye ingredients and commercial dyes have been shown to give rise, in the urine of treated rats, to metabolites which are also mutagenic in bacteria. Expand
Relationships between the Chemical Structure and Mutagenic Activity of Monocyclic Aromatic Amines
Hundreds of compounds have now been tested for mutagenic activity in many laboratories throughout the world. Mutagenicity tests are performed to validate a variety of assay systems and to contributeExpand
Phenytoin sodium Chem. Abstr. Serv. Reg. No.: 630-93-3 Deleted CAS Reg. Nos: 143-75-9; 1421-15-4; 8017-52-5 Chem. Abstr. Name: 5,5-Diphenyl-2,4-imidazolidinedione, monosodium salt ¡UPAC SystematicExpand


Metabolic activation of 2,4-diamino-anisole, a hair-dye component--I. Role of cytochrome p-450 metabolism in mutagenicity in vitro.
It is suggested that metabolic activation of 2, 4-diaminoanisole to the hydroxylamine(s) may be the underlying reaction for the formation of mutagenic intermediates. Expand
Mutagenic activation of N-hydroxy-2-acetylaminofluorene in the Salmonella test system: the role of deacetylation by liver and kidney fractions from mouse and rat.
The data indicate that deacetylation is the most important step in the mutagenic activation of N-hydroxy-2-acetylaminofluorene by mouse and rat liver and kidney fractions and that the arylnitrenium ion is the electrophilic species interacting with the bacterial DNA, resulting in the frameshift mutation. Expand
Mutagenesis of certain activated carcinogens in vitro associated with genetically mediated increases in monooxygenase activity and cytochrome P 1-450.
A bacterial mutagenesis assay and genetic differences in microsomal CO-binding cytochromes were combined in vitro to evaluate the metabolic activation of several known carcinogens to frameshiftExpand
Metabolism of the dyestuff intermediate 2,4-diaminoanisole in the rat.
1.2HCl administered intraperitoneally to rats is excreted chiefly via the urine (79 and 85% of the dose in 24 and 48 h, respectively) and the isotope in the faeces was 2,4-Diamino[ring-U-14C]anisole. Expand
Genetic differences in 2-acetylaminofluorene mutagenicity in vitro associated with mouse hepatic aryl hydrocarbon hydroxylase activity induced by polycyclic aromatic compounds.
It is suggested that the rate-limiting step of 2-acetylaminofluorene mutagenesis is its activation by cytochrome P1450 to the N -hydroxy derivative, which is metabolized further to a much more mutagenic intermediate by a reaction independent of cy tochrome P 1450-possibly a deacetylation reaction. Expand
Hair dyes are mutagenic: identification of a variety of mutagenic ingredients.
It is shown here that 89% (150/169) of commercial oxidative-type (hydrogen peroxide) hair dye formulations are mutagenic in this test, a sensitive bacterial test for detecting carcinogens as mutagens. Expand
Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test.
The methods described include the standard plate test, the use and storage of the bacterial tester strains, preparation and use of the liver homogenates, and the methods of inducing the rats for elevated microsomal enzyme activity. Expand
Protein measurement with the Folin phenol reagent.
Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein. Expand