Mutagenicity in Escherichia coli of the major DNA adduct derived from the endogenous mutagen malondialdehyde.

  title={Mutagenicity in Escherichia coli of the major DNA adduct derived from the endogenous mutagen malondialdehyde.},
  author={Stephen P. Fink and G. Ramachandra Reddy and Lawrence J Marnett},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={94 16},
The spectrum of mutations induced by the naturally occurring DNA adduct pyrimido[1,2-alpha]purin-10(3H)-one (M1G) was determined by site-specific approaches using M13 vectors replicated in Escherichia coli. M1G was placed at position 6256 in the (-)-strand of M13MB102 by ligating the oligodeoxynucleotide 5'-GGT(M1G)TCCG-3' into a gapped-duplex derivative of the vector. Unmodified and M1G-modified genomes containing either a cytosine or thymine at position 6256 of the (+)-strand were transformed… CONTINUE READING
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