Mutagenic specificity of reductively activated 1-nitropyrene in Escherichia coli.

@article{Malia1995MutagenicSO,
  title={Mutagenic specificity of reductively activated 1-nitropyrene in Escherichia coli.},
  author={Scuth Malia and Ashis K Basu},
  journal={Biochemistry},
  year={1995},
  volume={34 1},
  pages={
          96-104
        }
}
1-Nitropyrene (1-NP), the predominant nitropolycyclic hydrocarbon found in diesel exhaust, is a mutagen and tumorigen. Nitroreduction is a major pathway by which 1-NP is metabolized. In order to study the distribution of DNA adducts and the mutational specificity of reductively activated 1-NP, single stranded M13mp18 DNA was treated with N-hydroxyl-1-aminopyrene generated in situ to give > 95% of one major adduct, N-(deoxyguanosin-8-yl)-1-aminopyrene. A primer was annealed to DNA containing… CONTINUE READING
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