Mutagenic analysis of potato virus X movement protein (TGBp1) and the coat protein (CP): in vitro TGBp1-CP binding and viral RNA translation activation.

@article{Zayakina2008MutagenicAO,
  title={Mutagenic analysis of potato virus X movement protein (TGBp1) and the coat protein (CP): in vitro TGBp1-CP binding and viral RNA translation activation.},
  author={Olga V Zayakina and Marina V Arkhipenko and Stanislav V Kozlovsky and Nikolai Nikitin and Alexander Smirnov and Petri Susi and Nina P. Rodionova and Olga Karpova and Joseph Atabekov},
  journal={Molecular plant pathology},
  year={2008},
  volume={9 1},
  pages={37-44}
}
Previously, we have shown that encapsidated Potato virus X (PVX) RNA was non-translatable in vitro, but could be converted into a translatable form by binding of the PVX movement protein TGBp1 to one end of the virion or by coat protein (CP) phosphorylation. Here, a mutagenic analysis of PVX CP and TGBp1 was used to identify the regions involved in TGBp1-CP binding and translational activation of PVX RNA by TGBp1. It was found that the C-terminal (C-ter) 10/18 amino acids region was not… CONTINUE READING
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