Mutagenesis of bacteriophage T7 in vitro by incorporation of O6-methylguanine during DNA synthesis.

@article{Dodson1982MutagenesisOB,
  title={Mutagenesis of bacteriophage T7 in vitro by incorporation of O6-methylguanine during DNA synthesis.},
  author={Linda A. Dodson and Robert S. Foote and Sankar Mitra and Warren Masker},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1982},
  volume={79 23},
  pages={7440-4}
}
An in vitro system in which bacteriophage T7 DNA is replicated and efficiently packaged into procapsids to form viable phage has been used to examine mutagenesis. The fidelity of replication was assayed both by measuring reversion of an amber mutation in an essential gene and by generation of temperature-sensitive mutants among the phage produced in vitro. Under standard reaction conditions, the fidelity of DNA replication is about equal to that normally found in vivo. However, when O6… CONTINUE READING