Mutagenesis of Coronavirus nsp14 Reveals Its Potential Role in Modulation of the Innate Immune Response.

@article{Bcares2016MutagenesisOC,
  title={Mutagenesis of Coronavirus nsp14 Reveals Its Potential Role in Modulation of the Innate Immune Response.},
  author={Martina B{\'e}cares and Alejandro Pascual-Iglesias and Aitor Nogales and Isabel Sola and Luis Enjuanes and Sonia Z{\'u}{\~n}iga},
  journal={Journal of virology},
  year={2016},
  volume={90 11},
  pages={5399-5414}
}
UNLABELLED Coronavirus (CoV) nonstructural protein 14 (nsp14) is a 60-kDa protein encoded by the replicase gene that is part of the replication-transcription complex. It is a bifunctional enzyme bearing 3'-to-5' exoribonuclease (ExoN) and guanine-N7-methyltransferase (N7-MTase) activities. ExoN hydrolyzes single-stranded RNAs and double-stranded RNAs (dsRNAs) and is part of a proofreading system responsible for the high fidelity of CoV replication. nsp14 N7-MTase activity is required for viral… CONTINUE READING