Mutagenesis mediated by triple helix-forming oligonucleotides conjugated to psoralen: effects of linker arm length and sequence context.

@article{Raha1998MutagenesisMB,
  title={Mutagenesis mediated by triple helix-forming oligonucleotides conjugated to psoralen: effects of linker arm length and sequence context.},
  author={M Raha and Laurent Lacroix and Peter M. Glazer},
  journal={Photochemistry and photobiology},
  year={1998},
  volume={67 3},
  pages={289-94}
}
Targeted mutagenesis and gene knock-out can be mediated by triple helix-forming oligonucleotides (TFO) linked to mutagenic agents, such as psoralen. However, this strategy is limited by the availability of homopurine/homopyrimidine stretches at or near the target site because such sequences are required for high-affinity triplex formation. To overcome this limitation, we have tested TFO conjugated to psoralen via linker arms of lengths varying from 2 to 86 bonds, thereby designed to deliver the… CONTINUE READING

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