Mutagenesis in Escherichia coli lacking catalase.

  title={Mutagenesis in Escherichia coli lacking catalase.},
  author={Nieves Abril and Carmen Pueyo},
  journal={Environmental and molecular mutagenesis},
  volume={15 4},
Escherichia coli K-12 strains completely lacking catalase activity due to mutations in katG, katE, and katF genes were constructed in order to assess the role of hydrogen peroxide in mutagenesis. Mutagenesis was monitored by selecting forward mutations to L-arabinose resistance. Lethality was measured at experimental conditions equivalent to those of the mutant yield by using a mixed culture of pairs of isogenic strains distinguished by their differential nutritional requirements. Deficiency in… CONTINUE READING