Mutagenesis ’ ’ by peptide aptamers identifies genetic network members and pathway connections

@inproceedings{EWCGPMutagenesis,
  title={Mutagenesis ’ ’ by peptide aptamers identifies genetic network members and pathway connections},
  author={EWCGP and GPCKM}
}
  • EWCGP, GPCKM
We selected peptide aptamers from combinatorial libraries that disrupted cell-cycle arrest caused by mating pheromone in yeast. We used these aptamers as baits in two-hybrid hunts to identify genes involved in cell-cycle arrest. These experiments identified genes known to function in the pathway, as well as a protein kinase, the CBK1 product, whose function was not known. We used a modified two-hybrid system to identify specific interactions disrupted by these aptamers. These experiments… CONTINUE READING

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Geyer et al

  • C. Dietzel, Kurjan, 50 J.Cell, 1001–1010. 8572 Genetics
  • Proc. Natl. Acad. Sci. USA 96
  • 1999
1 Excerpt

Experiments in Molecular Genetics (Cold

  • J. H. Miller
  • 1972
1 Excerpt

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