Mutagenesis, cloning and complementation analysis of C4-dicarboxylate transport genes from Rhodobacter capsulatus.

@article{Hamblin1990MutagenesisCA,
  title={Mutagenesis, cloning and complementation analysis of C4-dicarboxylate transport genes from Rhodobacter capsulatus.},
  author={Mark J. Hamblin and Jonathan G. Shaw and J P Curson and DeirdreA. Kelly},
  journal={Molecular microbiology},
  year={1990},
  volume={4 9},
  pages={
          1567-74
        }
}
Transposon mutagenesis was used to isolate insertion mutants of the photosynthetic bacterium Rhodobacter capsulatus which were unable to grow under aerobic conditions in the dark on malate, succinate or fumarate as sole carbon sources. Of five mutants isolated, all were deficient in C4-dicarboxylate transport. However, these mutants were still capable of photoheterotrophic growth, although at a slower rate than the wild type, on malate and succinate (but not fumarate). The mutated locus… CONTINUE READING

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