MutS as a tool for mutation detection.

  title={MutS as a tool for mutation detection.},
  author={Anna Stanisławska-Sachadyn and Paweł Sachadyn},
  journal={Acta biochimica Polonica},
  volume={52 3},
MutS, a DNA mismatch-binding protein, seems to be a promising tool for mutation detection. We present three MutS based approaches to the detection of point mutations: DNA retardation, protection of mismatched DNA against exonuclease digestion, and chimeric MutS proteins. DNA retardation in polyacrylamide gels stained with SYBR-Gold allows mutation detection using 1-3 microg of Thermus thermophilus his6-MutS protein and 50-200 ng of a PCR product. The method enables the search for a broad range… CONTINUE READING


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