Muscle lactate transport studied in sarcolemmal giant vesicles.

  title={Muscle lactate transport studied in sarcolemmal giant vesicles.},
  author={Carsten Juel},
  journal={Biochimica et biophysica acta},
  volume={1065 1},
Lactate transport was studied in giant (median diameter 6.3 microns) sarcolemmal vesicles obtained by collagenase treatment of rat skeletal muscle. The lactate transport displayed stereospecificity, had a high temperature coefficient, and could be inhibited up to 90% with known transport inhibitors (PCMBS and cinnamate). In equilibrium exchange experiments, the L-lactate flux demonstrated saturation kinetics with Km = 23.7 mM and Vmax = 108 pmol cm-2 s-1. With lactate present on only one side… CONTINUE READING


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