Muscarinic receptors coupled to inositol phospholipid breakdown in human SH-SY5Y neuroblastoma cells: Effect of retinoic acid-induced differentiation.

In human SH-SY5Y neuroblastoma cells, carbachol stimulates inositol phospholipid breakdown with an EC(50) value of 18 ?M. The response is prevented by pirenzepine, with a Hill coefficient of 0.57 and an IC(50) value of 0.15 ?M. A Gpp[NH]p-stimulated [(3)H]phosphatidylinositol-4,5-bisphosphate hydrolysing activity was demonstrated in SH-SY5Y membrane… (More)