Muscarinic receptor-induced calcium responses in astroglia.

  title={Muscarinic receptor-induced calcium responses in astroglia.},
  author={Michelle C. Catlin and Terrance J Kavanagh and Lucio Guido Costa},
  volume={41 2},
BACKGROUND The objective of this study was to characterize and quantitate the calcium responses to cholinergic stimulation in individual primary rat cortical astrocytes and human 132 1N1 astrocytoma cells. Materials and Methods The fluorescent calcium probe Indo-1 AM and an attached cell analysis and sorting (ACAS) instrument were used to quantitate calcium responses in these cells. RESULTS A concentration-dependent response to carbachol was seen in both cell types. However, carbachol was… CONTINUE READING