Muscarinic Agonists Activate Ca2+ Store-operated and -independent Ionic Currents in Insulin-secreting HIT-T15 Cells and Mouse Pancreatic β-Cells

@article{Mears2003MuscarinicAA,
  title={Muscarinic Agonists Activate Ca2+ Store-operated and -independent Ionic Currents in Insulin-secreting HIT-T15 Cells and Mouse Pancreatic β-Cells},
  author={David Mears and Charles L Zimliki},
  journal={The Journal of Membrane Biology},
  year={2003},
  volume={197},
  pages={59-70}
}
The neurotransmitter acetylcholine, a muscarinic receptor agonist, augments glucose-induced insulin secretion from pancreatic β-cells by depolarizing the membrane to enhance voltage-gated Ca2+ influx. To clarify the electrical events involved in this process, we measured ionic currents from a clonal β-cell line (HIT-T15) and mouse pancreatic β-cells. In whole-cell recordings, the muscarinic agonist carbachol (CCh) dose-dependently and reversibly activated a voltage-independent, nonselective… CONTINUE READING