Mus81 and converging forks limit the mutagenicity of replication fork breakage

@article{Mayle2015Mus81AC,
  title={Mus81 and converging forks limit the mutagenicity of replication fork breakage},
  author={Ryan Mayle and Ian M. Campbell and Christine R. Beck and Yang Yu and Marenda A. Wilson and Chad A. Shaw and Lotte Bjergbaek and James R Lupski and Grzegorz Ira},
  journal={Science},
  year={2015},
  volume={349},
  pages={742-747}
}
Most spontaneous DNA double-strand breaks (DSBs) result from replication-fork breakage. Break-induced replication (BIR), a genome rearrangement–prone repair mechanism that requires the Pol32/POLD3 subunit of eukaryotic DNA Polδ, was proposed to repair broken forks, but how genome destabilization is avoided was unknown. We show that broken fork repair initially uses error-prone Pol32-dependent synthesis, but that mutagenic synthesis is limited to within a few kilobases from the break by Mus81… CONTINUE READING