Murine spinal cord explants: a model for evaluating axonal growth and myelination in vitro.

@article{Thomson2006MurineSC,
  title={Murine spinal cord explants: a model for evaluating axonal growth and myelination in vitro.},
  author={Christine E. Thomson and Anne M Hunter and Ian R. Griffiths and Julia M Edgar and Mailis C. McCulloch},
  journal={Journal of neuroscience research},
  year={2006},
  volume={84 8},
  pages={1703-15}
}
In vitro models of myelinating central nervous system axons have mainly been of two types, organotypic or dissociated. In organotypic cultures, the tissue fragment is thick and usually requires sectioning (physically or optically) before visual examination. In dissociated cultures, tissue is dispersed across the culture surface, making it difficult to measure the extent of myelinated fiber growth. We aimed to develop a method of culturing myelinated CNS fibers in defined medium that could be 1… CONTINUE READING