Murine passive cutaneous anaphylaxis test (PCA) for the 'all or none' determination of allergenicity of bovine whey proteins and peptides.

@article{Poulsen1987MurinePC,
  title={Murine passive cutaneous anaphylaxis test (PCA) for the 'all or none' determination of allergenicity of bovine whey proteins and peptides.},
  author={Otto Melchior Poulsen and Jann Hau},
  journal={Clinical allergy},
  year={1987},
  volume={17 1},
  pages={75-83}
}
A passive cutaneous anaphylaxis test (PCA) for determination of the allergenicity of bovine whey proteins and peptides was developed in mice. Antisera against whey proteins raised in rabbits and in mice, using a procedure for high IgE titre mixed with Freund's incomplete adjuvant, were applied intradermally, and various whey proteins and whey protein hydrolysates were tested for positive PCA reaction. Unhydrolysed whey and peptides larger than 6500 Da were found to react positively, peptides… CONTINUE READING