Murine embryonic stem cell differentiation is promoted by SOCS-3 and inhibited by the zinc finger transcription factor Klf4.

@article{Li2005MurineES,
  title={Murine embryonic stem cell differentiation is promoted by SOCS-3 and inhibited by the zinc finger transcription factor Klf4.},
  author={Yanjun Li and J. McClintick and L. Zhong and H. Edenberg and M. Yoder and R. Chan},
  journal={Blood},
  year={2005},
  volume={105 2},
  pages={
          635-7
        }
}
Embryonic stem (ES) cells homozygous for a Shp-2 mutation (Shp-2(Delta46-110)) demonstrate leukemia inhibitory factor (LIF) hypersensitivity and increased LIF-stimulated phosphorylation of signal transducer and activator of transcription (STAT3). We hypothesized that LIF-responsive genes in Shp-2(Delta46-110) cells would represent potential candidates for molecules vital for ES cell self-renewal. Using microarray analysis, we detected 41 genes whose expression was modified by LIF in Shp-2… Expand
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CHIR99021 enhances Klf4 Expression through β-Catenin Signaling and miR-7a Regulation in J1 Mouse Embryonic Stem Cells
  • Zhiying Ai, Jingjing Shao, +6 authors Zekun Guo
  • Biology, Medicine
  • PloS one
  • 2016
TLDR
DNA microarray analysis revealed that CHIR-treatment promoted pluripotency-maintaining transcription factors and repressed germ layer specification markers and several microRNAs that targeted the 3′-UTR of Klf4 mRNA were identified, suggesting thatCHIR enhances Klf 4 expression by repressing miR-7a expression or canonical Wnt pathway activation. Expand
Krüppel-like factor 4, a “pluripotency transcription factor” highly expressed in male postmeiotic germ cells, is dispensable for spermatogenesis in the mouse
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Despite its important roles in several significant biological settings, deletion of Klf4 in germ cells did not impair spermiogenesis and the lack of KLF4 alone does not prevent complete spermatogenesis. Expand
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