Murine bone marrow-derived macrophages constitute feeder cells for human B cell hybridomas.

@article{Hoffmann1996MurineBM,
  title={Murine bone marrow-derived macrophages constitute feeder cells for human B cell hybridomas.},
  author={Petra Hoffmann and Mar{\'i}a Jim{\'e}nez-D{\'i}az and J. Weckesser and Wolfgang G. Bessler},
  journal={Journal of immunological methods},
  year={1996},
  volume={196 1},
  pages={
          85-91
        }
}
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13 Citations
Background Citations
2
Methods Citations
2
Results Citations
1

13 Citations

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Immunostimulation by bacterial components: I. Activation Of macrophages and enhancement of genetic immunization by the lipopeptide P3CSK4.
Lipopeptide adjuvants: monitoring and comparison of P3CSK4- and LPS-induced gene transcription.
Immunostimulation by the synthetic lipopeptide P3CSK4: TLR4‐independent activation of the ERK1/2 signal transduction pathway in macrophages
TLDR
The signal transduction pathways involved in leucocyte activation by the synthetic lipopeptide N‐palmitoyl‐S‐[2,3‐bis(palMIToyloxy)‐(2R,S)‐propyl]‐(R)‐cysteinyl‐seryl‐(lysyl)3‐lysine (P3CSK4) are investigated.
Comparison of beneficial factors for corneal wound-healing of rat mesenchymal stem cells and corneal limbal stem cells on the xenogeneic acellular corneal matrix in vitro
TLDR
Under the ACM microenvironment, the MSCs presented beneficial factors for corneal recovery comparable to those presented by corneAL LSCs, indicating that MSC’s, when combined with an ACM, may compose a competent corNEal substitute for healing cornea wounds.
MONOCLONAL ANTIBODY PRODUCTION
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In 1975, Georges Köhler and Cesar Milstein devised a method for preparing monoclonal antibody, which quickly became one of immunology’s key technologies.
The bacterial extract Broncho-Vaxom protects against respiratory infections-in vivo and in vitro studies
TLDR
In vitro analysis of biological material of mouse treated with OM-85 BV revealed an increase of bacteria specific IgA in supernatants of Peyer's patches and mesenteric lymph nodes derived cell cultures, which demonstrates the antimicrobial activity of Broncho-Vaxom.
Exploring the Native Human Antibody Repertoire to Create Antiviral Therapeutics
TLDR
This review discusses native human antibodies and ongoing improvements in cloning methods that should facilitate the creation of novel, potent antiviral therapeutics obtained from the native human antibody repertoire.
...
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References

SHOWING 1-10 OF 27 REFERENCES
Induction of tumor cytotoxicity in murine bone marrow-derived macrophages by two synthetic lipopeptide analogues.
TLDR
The results demonstrate that synthetic lipopeptides are potent activators for murine BMDM and may therefore prove to be an important tool for the elucidation of the role of macrophages in the host defence mechanisms against tumor cells.
Mitomycin C-treated 3T3/B (3T3/A31) cell feeder layers in hybridoma technology.
The high efficiency, human B cell immortalizing heteromyeloma CB-F7. Production of human monoclonal antibodies to human immunodeficiency virus.
Preparation of human and murine monoclonal antibodies: antigens combined with or conjugated to lipopeptides constitute potent immunogens for in vitro and in vivo immunizations.
TLDR
The novel immunization procedure offers reproducibility, high antibody titers often after one immunization, lack of toxicity of the adjuvants, easy chemical preparation of the conjugates in mg amounts, and the applicability of the Conjugates for screening for the antibodies obtained.
Sequential expression of functions during macrophage differentiation in murine bone marrow liquid cultures
TLDR
It is suggested that the phenotypic expression of functions during differentiation is the basis for the functional heterogeneity of macrophages.
Hybridoma cell lines secreting monoclonal antibodies against foot-and-mouth disease virus (FMDV). II. Cloning conditions.
The use of rat mixed-thymocyte culture-conditioned medium for hybridoma production, cloning and revival.
Production of human monoclonal antibodies to B lymphocyte cell surface antigens by in vitro immunization and human-human hybridoma technology.
TLDR
The data suggest that in vitro immunization using the protocol outlined can increase the frequency of some antibodies, but that they may represent a very primitive lineage which does not have the specificity and affinity required to prove useful as diagnostic or therapeutic agents.
Production of anti-tumor human monoclonal antibodies using different approaches.
TLDR
The production of anti-tumor human monoclonal antibodies (MAbs) by human-human or human-mouse hybridoma technology was studied and supernatants from these hybridomas all reacted with breast cancer cell lines but not with gastric cancer cell Lines.
Endothelial cell growth supplement: a cell cloning factor that promotes the growth of monoclonal antibody producing hybridoma cells.
...
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