Murine C4b-binding protein. Mapping of the ligand binding site and the N-terminus of the pre-protein.

@article{Ogata1993MurineCP,
  title={Murine C4b-binding protein. Mapping of the ligand binding site and the N-terminus of the pre-protein.},
  author={Ronald T. Ogata and Patricia I Mathias and Bonnie M. Bradt and Neil R. Cooper},
  journal={Journal of immunology},
  year={1993},
  volume={150 6},
  pages={2273-80}
}
We have constructed cell surface-bound forms of murine C4b-binding protein (mC4BP) that allowed us to monitor the binding of mC4BP to C4b with relatively simple erythrocyte rosette assays. We used two types of surface-bound mC4BP: one in which segments of mC4BP were fused directly to a peptide containing the transmembrane and cytoplasmic domains of human complement receptor CR2 (BPR1-type); and a second in which the same segments were fused to a longer peptide containing the five membrane… CONTINUE READING

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