Murine 3T3-L1 Adipocyte Cell Differentiation Model: Validated Reference Genes for qPCR Gene Expression Analysis

@inproceedings{Arsenijevic2012Murine3A,
  title={Murine 3T3-L1 Adipocyte Cell Differentiation Model: Validated Reference Genes for qPCR Gene Expression Analysis},
  author={Tatjana Arsenijevic and Françoise Gr{\'e}goire and Val{\'e}rie Delforge and Christine Delporte and Jason Perret},
  booktitle={PloS one},
  year={2012}
}
BACKGROUND Analysis of gene expression at the mRNA level, using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), mandatorily requires reference genes (RGs) as internal controls. However, increasing evidences have shown that RG expression may vary considerably under experimental conditions. We sought for an appropriate panel of RGs to be used in the 3T3-L1 cell line model during their terminal differentiation into adipocytes. To this end, the expression levels of… CONTINUE READING
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