Muralytic activity of Micrococcus luteus Rpf and its relationship to physiological activity in promoting bacterial growth and resuscitation.

@article{Mukamolova2006MuralyticAO,
  title={Muralytic activity of Micrococcus luteus Rpf and its relationship to physiological activity in promoting bacterial growth and resuscitation.},
  author={Galina V. Mukamolova and Alexey G. Murzin and Elena G. Salina and Galina R. Demina and Douglas B. Kell and Arseny S. Kaprelyants and Michael Young},
  journal={Molecular microbiology},
  year={2006},
  volume={59 1},
  pages={84-98}
}
The culturability of several actinobacteria is controlled by resuscitation-promoting factors (Rpfs). These are proteins containing a c. 70-residue domain that adopts a lysozyme-like fold. The invariant catalytic glutamate residue found in lysozyme and various bacterial lytic transglycosylases is also conserved in the Rpf proteins. Rpf from Micrococcus luteus, the founder member of this protein family, is indeed a muralytic enzyme, as revealed by its activity in zymograms containing M. luteus… CONTINUE READING
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