Multivalent ligand-receptor binding interactions in the fibroblast growth factor system produce a cooperative growth factor and heparin mechanism for receptor dimerization.

@article{Pantoliano1994MultivalentLB,
  title={Multivalent ligand-receptor binding interactions in the fibroblast growth factor system produce a cooperative growth factor and heparin mechanism for receptor dimerization.},
  author={Michael W Pantoliano and Robert A. Horlick and Barry A. Springer and Drew E Van Dyk and T Tobery and D R Wetmore and James D. Lear and A. Nahapetian and Jodi D Bradley and W. P. Sisk},
  journal={Biochemistry},
  year={1994},
  volume={33 34},
  pages={10229-48}
}
The binding interactions for the three primary reactants of the fibroblast growth factor (FGF) system, basic FGF (bFGF), an FGF receptor, FGFR1, and the cofactor heparin/heparan sulfate (HS), were explored by isothermal titrating calorimetry, ultracentrifugation, and molecular modeling. The binding reactions were first dissected into three binary reactions: (1) FGFR1 + bFGF<==>FGFR1/bFGF, K1 = 41 (+/- 12) nM; (2) FGFR1 + HS<==>FGFR1/HS, K2 = 104 (+/- 17) microM; and (3) bFGF + HS<==>bFGF/HS, K3… CONTINUE READING

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