Multitypic hepatitis C virus infection identified by real-time nucleotide sequencing of minority genotypes.

@article{Buckton2006MultitypicHC,
  title={Multitypic hepatitis C virus infection identified by real-time nucleotide sequencing of minority genotypes.},
  author={Andrew J. Buckton and S L Ngui and Catherine Arnold and Katie Boast and Joanne Kovacs and Paul E. Klapper and Bharat H. Patel and Imad Ibrahim and S Rangarajan and Mary Elizabeth Ramsay and Chong-Gee Teo},
  journal={Journal of clinical microbiology},
  year={2006},
  volume={44 8},
  pages={2779-84}
}
The prevalence of concurrent multitypic hepatitis C virus (HCV) infection is uncertain. A sensitive and specific approach to identifying minority HCV genotypes in blood is presented. Following serum extraction and reverse transcription PCR to amplify cDNA originating from the viral 5' noncoding region, the amplified product mixture was treated with genotype-specific restriction endonuclease to digest the dominant genotype. Residual amplicons were subjected to PCR cloning and then to real-time… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 25 references

Similar Papers

Loading similar papers…