Multitemperature single-strand conformation polymorphism.

  title={Multitemperature single-strand conformation polymorphism.},
  author={Radosław Kaczanowski and Lech Trzeciak and Krzysztof Kucharczyk},
  volume={22 16},
Changes of gel temperature during single-strand conformation polymorphism (SSCP) electrophoresis increase the sensitivity of mutation detection in polymerase chain reaction (PCR) products and significantly reduce the overall time and costs of analysis. Based on these findings, a new method for single nucleotide polymorphism (SNP) and point mutation detection--multitemperature single-strand conformation polymorphism (MSSCP) was devised. In order to control the gel temperature with 0.1 degrees C… CONTINUE READING