Multitarget super-resolution microscopy with high-density labeling by exchangeable probes

@article{Kiuchi2015MultitargetSM,
  title={Multitarget super-resolution microscopy with high-density labeling by exchangeable probes},
  author={Tai Kiuchi and Makio Higuchi and Akihiro Takamura and Masahiro Maruoka and Naoki Watanabe},
  journal={Nature Methods},
  year={2015},
  volume={12},
  pages={743-746}
}
We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment–based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the… CONTINUE READING