Multispectral labeling technique to map many neighboring axonal projections in the same tissue

@article{Tsuriel2015MultispectralLT,
  title={Multispectral labeling technique to map many neighboring axonal projections in the same tissue},
  author={Shlomo Tsuriel and Sagi Gudes and R. W. Draft and Alexander M. Binshtok and Jeff William Lichtman},
  journal={Nature Methods},
  year={2015},
  volume={12},
  pages={547-552}
}
We describe a method to map the location of axonal arbors of many individual neurons simultaneously via the spectral properties of retrogradely transported dye-labeled vesicles. We inject overlapping regions of an axon target area with three or more different colored retrograde tracers. On the basis of the combinations and intensities of the colors in the… CONTINUE READING