Multiscale tissue engineering for liver reconstruction

@article{Sudo2014MultiscaleTE,
  title={Multiscale tissue engineering for liver reconstruction},
  author={Ryo Sudo},
  journal={Organogenesis},
  year={2014},
  volume={10},
  pages={216 - 224}
}
  • R. Sudo
  • Published 5 February 2014
  • Biology
  • Organogenesis
The liver is a target of in vitro tissue engineering despite its capability to regenerate in vivo. The construction of liver tissues in vitro remains challenging. In this review, conventional 3D cultures of hepatocytes are first discussed. Recent advances in the 3D culturing of liver cells are then summarized in the context of in vitro liver tissue reconstruction at the micro- and macroscales. The application of microfluidics technology to liver tissue engineering has been introduced as a… 

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References

SHOWING 1-10 OF 94 REFERENCES

Human-Scale Whole-Organ Bioengineering for Liver Transplantation: A Regenerative Medicine Approach

TLDR
The decellularization of porcine livers is described to generate liver constructs at a scale that can be clinically relevant and provides proof-of-principle for the generation of a human-sized, three-dimensional organ scaffold as a potential structure for human liver grafts reconstruction for transplantation to treat liver disease.

Assessing porcine liver-derived biomatrix for hepatic tissue engineering.

TLDR
Data indicate that decellularized, porcine, liver-derived biomatrix (LBM) may be a favorable alternative to existing scaffolds for tissue engineering in that it is bioresorbable, can be easily manipulated, and supports long-term hepatocellular functions in vitro.

Construction of a Portal Implantable Functional Tissue-Engineered Liver Using Perfusion-Decellularized Matrix and Hepatocytes in Rats

TLDR
The TEL is proposed as a state-of-the-art substitute for whole-liver transplantation and as a proof of concept for the technology that will eventually allow for the transplantation of a reconstituted liver.

The use of whole organ decellularization for the generation of a vascularized liver organoid

TLDR
Three‐dimensional, naturally derived scaffolds with an intact vascular tree are fabricated and may provide the necessary tools to produce the first fully functional bioengineered livers for organ transplantation and drug discovery.

Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds.

TLDR
The fundamental concepts of whole-organ engineering, including characterization of the extracellular matrix as a scaffold, methods for decellularization of vascular organs, potential cells to reseed such a scaffolds, techniques for the recellularization process and important aspects regarding bioreactor design to support this approach are described.

Perfused multiwell plate for 3D liver tissue engineering.

TLDR
A bioreactor is developed that fosters maintenance of 3D tissue cultures under constant perfusion and multiple bioreactors are integrated into an array in a multiwell plate format to capture the complexity of in vivo tissue and organ behaviors.

Decellularization for whole organ bioengineering.

TLDR
Techniques used to decellularize whole organs such as the heart, lung, liver and kidney are described and possible methods for using these matrices for whole organ engineering are described.

Use of decellularized porcine liver for engineering humanized liver organ.

Whole-organ re-engineering: a regenerative medicine approach in digestive surgery for organ replacement

TLDR
Organ decellularization technology is applied to produce whole organ-derived scaffolds by removing cellular content while retaining all the necessary vascular and structural cues of the native organ in the field of digestive surgery.

Microfabrication Technology for Vascularized Tissue Engineering

TLDR
The concept of microfabricated scaffolds is introduced, with the goal of producing organ templates with feature resolution of 1 micron, well in excess of that necessary to fashion the capillaries which comprise the microcirculation of the organ.
...