Multipronged approach to managing beta-glucan contaminants in the downstream process: control of raw materials and filtration with charge-modified nylon 6,6 membrane filters.

@article{Gefroh2013MultiprongedAT,
  title={Multipronged approach to managing beta-glucan contaminants in the downstream process: control of raw materials and filtration with charge-modified nylon 6,6 membrane filters.},
  author={Eva Gefroh and Art Hewig and Ganesh Vedantham and Megan McClure and Alla Krivosheyeva and Ajay R Lajmi and Yuefeng Lu},
  journal={Biotechnology progress},
  year={2013},
  volume={29 3},
  pages={672-80}
}
(1→3)-β-D-Glucans (beta-glucans) have been found in raw materials used in the manufacture of recombinant therapeutics. Because of their biological activity, beta-glucans are considered process contaminants and consequently their level in the product needs to be controlled. Although beta-glucans introduced into the cell culture process can readily be removed by bind-and-elute chromatography process steps, beta-glucans can also be introduced into the purification process through raw materials… CONTINUE READING

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