Multiprimed cDNA synthesis followed by PCR is the most suitable method for Epstein-Barr virus transcript analysis in small lymphoma biopsies.

@article{Brink1997MultiprimedCS,
  title={Multiprimed cDNA synthesis followed by PCR is the most suitable method for Epstein-Barr virus transcript analysis in small lymphoma biopsies.},
  author={Antoinette Atp Brink and J. J. Oudejans and Mehdi Jiwa and Jan M. M. Walboomers and Chris Jlm Meijer and Adriaan J. C. van den Brule},
  journal={Molecular and cellular probes},
  year={1997},
  volume={11 1},
  pages={
          39-47
        }
}
In this study, the reverse transcriptase-polymerase chain reaction (RT-PCR) for the reliable detection of multiple Epstein-Barr virus (EBV) transcripts was optimized and subsequently evaluated on lymphoma specimens. Since often only small lymphoma biopsies are available for analysis of EBV transcripts, several RT-protocols to generate cDNA from multiple targets were applied. These were multi-primer, oligo-dT primed and random hexamer primed cDNA synthesis. Multi-primer cDNA synthesis appeared… CONTINUE READING
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