Multipotential differentiation of human urine‐derived stem cells: Potential for therapeutic applications in urology

  title={Multipotential differentiation of human urine‐derived stem cells: Potential for therapeutic applications in urology},
  author={Shantaram Bharadwaj and Guihua Liu and Ying-ai Shi and Rongpei Wu and Bin Xia Yang and Tongchuan He and Yuxin Fan and Xinyan Lu and Xiaobo Zhou and Hong Liu and Anthony Atala and Jan Rohozinski and Yuanyuan Zhang},
  journal={STEM CELLS},
We sought to biologically characterize and identify a subpopulation of urine‐derived stem cells (USCs) with the capacity for multipotent differentiation. We demonstrated that single USCs can expand to a large population with 60–70 population doublings. Nine of 15 individual USC clones expressed detectable levels of telomerase and have long telomeres. These cells expressed pericyte and mesenchymal stem cell markers. Upon induction with appropriate media in vitro, USCs differentiated into bladder… 

Urine-Derived Stem Cells: Biological Characterization and Potential Clinical Applications

A subpopulation of urine- derived cells, termed urine-derived stem cells (USCs), possess stem cell capabilities, such as self-renewal and multipotential differentiation, and retain a normal karyotype in vitro even after several passages.

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The results of the study demonstrate that USCs possess similar biological characteristics with ASCs and have multilineage differentiation potential, and are a promising cell source for tissue engineering and regenerative medicine.

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UDSC biological characteristics and differentiation potential and their possible use, including the potential of UDSC-derived iPSC to be used in drug discovery and toxicology, as well as in regenerative medicine.

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This review will describe the isolation and characterization of urine-derived stem cells (USCs), and specifically present USC phenotype, which is not an object of consensus in the literature, as well as detail their differentiation capacity.

Skeletal myogenic differentiation of human urine‐derived cells as a potential source for skeletal muscle regeneration

The findings suggest that human urine‐derived stem cells, obtained through non‐invasive procedures, are able to differentiate into the Sk‐MC lineage in vitro and after being implanted in vivo, and might be a potential source for cell injection therapy in the use of skeletal muscle regeneration.

Comparison of the Proliferation and Differentiation Potential of Human Urine-, Placenta Decidua Basalis-, and Bone Marrow-Derived Stem Cells

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Differentiation Capacity of Human Urine-Derived Stem Cells to Retain Telomerase Activity

Overall, as a safe cell source, telomerase-positive USCs have a robust regenerative potential in cell proliferation and multipotent differentiation capacity.

Generation of Mesenchymal-Like Stem Cells From Urine in Pediatric Patients.

Urine-derived stem cells for potential use in bladder repair

Urine-derived stem cells (USCs) provide advantages for cell therapy and tissue engineering applications in bladder tissue repair because they originate from the urinary tract system and can be obtained via a noninvasive, simple, and low-cost approach and induced with high efficiency to differentiate into bladder cells.



Characterization of urine-derived stem cells obtained from upper urinary tract for use in cell-based urological tissue engineering.

Urothelial-differentiated uUSC possess expansion and differentiation capabilities, and can potentially be used as an alternative cell source in bladder tissue engineering for patients needing cystoplasty.

Differentiation of human bone marrow mesenchymal stem cells into bladder cells: potential for urological tissue engineering.

Smooth muscle- and urothelium-like cells derived from human BMSCs provide an alternative cell source for potential use in bladder tissue engineering.

Urine derived cells are a potential source for urological tissue reconstruction.

Evaluation of senescence in mesenchymal stem cells isolated from equine bone marrow, adipose tissue, and umbilical cord tissue.

The results demonstrate the limited passage numbers of subcultured BMSCs available for use in research and tissue engineering and suggest that adipose tissue and umbilical cord tissue may be preferable for tissue banking purposes.

Mesenchymal Stem Cells Can Be Differentiated Into Endothelial Cells In Vitro

The differentiation of expanded adult human MSCs into cells with phenotypic and functional features of endothelial cells are shown to provide new options for engineering of artificial tissues based on autologous M SCs and vascularized engineered tissues.

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG,

A biomimetic tissue from cultured normal human urothelial cells: analysis of physiological function.

This model represents major progress in developing a biomimetic human urothelial culture model to explore molecular and functional relationships in normal and dysfunctional bladder physiology.