Multipotent adult progenitor cell isolation and culture procedures.

  title={Multipotent adult progenitor cell isolation and culture procedures.},
  author={April Breyer and Nima Estharabadi and Masayuki Oki and Fernando Ulloa and Molly H Nelson-Holte and Laura Lien and Yuehua Jiang},
  journal={Experimental hematology},
  volume={34 11},
We describe methods for isolation of multipotent adult progenitor cells (MAPCs) from newborn to 6-week-old mice and rats. The maintenance of these cells, including their culture, media formulas, and quality control procedures, are also explained. Additionally, ways to identify MAPCs including their phenotype and morphology are discussed.