Multipoint imprinting analysis in sporadic colorectal cancers with and without microsatellite instability.

Abstract

Disrupted imprinting is implicated in certain tumorigenesis. Since aberrant methylation has been described for a majority of microsatellite instability (MSI)-positive sporadic colorectal cancers, we have investigated alteration to the imprinting in 55 sporadic colorectal cancers with or without MSI. Loss of imprinting (LOI) of IGF2 and PEG1/MEST was observed in 42% and 35% of informative cancers, respectively. H19 expression was not detected in 24% of informative cancers. SNRPN and NDN retained monoallelic expression in all the cancers examined. These findings indicate no simultaneous disruption of the imprinted genes. LOI of IGF2 and PEG1/MEST was also observed in colorectal mucosa from almost all the patients with LOI in tumor tissue. Moreover, MSI-positive colorectal cancers exhibit LOI of IGF2 with a high frequency compared to MSI-negative cancers (P=0.013). These observations, consistent with a previous report, establish an association between LOI of IGF2 and MSI in colorectal cancers and provide insight into susceptibility of tumor development.

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@article{Nishihara2000MultipointIA, title={Multipoint imprinting analysis in sporadic colorectal cancers with and without microsatellite instability.}, author={Souichi Nishihara and T Hayashida and Koichi Mitsuya and Thomas C. Schulz and Masahide Ikeguchi and Nobuaki Kaibara and Mitsuo Oshimura}, journal={International journal of oncology}, year={2000}, volume={17 2}, pages={317-22} }