Multiplicity Reactivation as a Test for Recombination Function

@article{Huskey1969MultiplicityRA,
  title={Multiplicity Reactivation as a Test for Recombination Function},
  author={Robert J. Huskey},
  journal={Science},
  year={1969},
  volume={164},
  pages={319 - 320}
}
  • R. Huskey
  • Published 18 April 1969
  • Biology, Medicine
  • Science
Multiplicity reactivation of bacteriophage inactivated by ultraviolet light is dependent on the recombination function of either the host bacterial cell or the infecting bacteriophage. Absence of both recombination systems leads to a loss of multiplicity reactivation. 
MULTIPLICITY REACTIVATION AND REPAIR OF LETHAL LESIONS INDUCED BY NITROUS ACID OR ULTRAVIOLET IRRADIATION IN BACTERIOPHAGE T4
An effort was made to define the gene functions that are in­ volved in the multiplicity reactivation of damaged T*f phage particles. First, wild type phage were either treated with nitrous acid orExpand
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TLDR
Results indicate the involvement of the E. coli recA gene product in normal T4 replication and multiplicity reactivation and show that irradiated phages can only be reactivated in recA + hosts, regardless of the bacteriophage genotype. Expand
Genetic evidence that the elevated levels of Escherichia coli helicase II antagonize recombinational DNA repair.
TLDR
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Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization.
TLDR
All of the nearly 200 deletion mutants studied are defective in the ability to promote recombination in recombinationless host bacteria and fall into three major classes with respect to their integration behavior in different host strains. Expand
Multiplicity reactivation and repair of nitrous acid-induced lesions in bacteriophage T4.
TLDR
The results indicate that, in wild-type single infections, about 20% of nitrous acid-induced lethal lesions may be repaired by a recombinational post-replication form of repair. Expand
Dark recovery of uv-irradiated phage TI. I. A minor recovery effect whose exclusion permits the study of survival kinetics under presumably repairless conditions.
  • W. Harm
  • Biology, Medicine
  • Mutation research
  • 1973
TLDR
The observed phage sensitivity and shape of the curve are compatible with the expectation for completely repairless conditions, and theoretical considerations make a phage REC repair mechanism seem likely. Expand
Evidence for repair of ultraviolet light-damaged herpes virus in human fibroblasts by a recombination mechanism.
TLDR
Experiments showed that the defective functions in these mutant host cells are not required for multiplicity-dependent repair or UV-stimulated viral recombination in herpes-infected cells, providing strong support for the model that genetic recombination between lethally damaged HSV-1 chromosomes can lead to the production of undamaged virus. Expand
DNA repair and the evolution of transformation IV. DNA damage increases transformation
TLDR
DNA damage directly increases rates of homologous recombination and transformation in B. subtilis, and the relevance of these results and recent results of other labs to the evolution of transformation are discussed. Expand
Multiplicity reactivation of alkylating agent damaged herpes simplex virus (type I) in human cells.
  • S. Das
  • Biology, Medicine
  • Mutation research
  • 1982
TLDR
Results indicate that HSV-1 infected human cells are capable of repairing chemically induced lesions by way of multiplicity reactivation, and no evidence for multiplier reactivation with HN2-treated virus could be obtained. Expand
Repair of psoralen-induced crosslinks in cells multiply infected with SV40
TLDR
A model is proposed for multiplicity reactivation which involves genetic recombination between damaged viral genomes in simian virus 40 DNA. Expand
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  • W. Harm
  • Biology, Medicine
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TLDR
The experimentally observed deviations from the expectations of the original recombination theory of multiplicity reactivation cannot be accounted for by the variability in the size of the host cells. Expand
A study of multiplicity-reactivation in the bacteriophage T4. I. Genetic and functional analysis of T4D-K12(lambda) complexes.
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Evidence is presented which suggests that the ultraviolet (UV) damages stimulate recombination in the neighborhood of their locations in the genetic structure of the phage, which appears to form a relatively large porportion of the sensitive target in MR. Expand
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Experiments on the mechanisms of DNA repair in Escherichia coli following exposure to irradiation or to mutagens, including alkylating agents and X-rays are reviewed and the role of DNA Repair in genetic recombination is reviewed. Expand
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TLDR
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