Multiplication of a human parasite (Leishmania donovani) in phagolysosomes of hamster macrophages in vitro.

@article{Chang1976MultiplicationOA,
  title={Multiplication of a human parasite (Leishmania donovani) in phagolysosomes of hamster macrophages in vitro.},
  author={Kwang Poo Chang and Dennis M. Dwyer},
  journal={Science},
  year={1976},
  volume={193 4254},
  pages={678-80}
}
Leishmania donovani, the etiological agent of human visceral leishmaniasis, was grown in hamster peritoneal macrophages in vitro. By electron microscopy, using a lysosomal marker, these parasitic protozoa were seen to multiply within host cell phagolysosomes. The survival mechanism of this intracellular parasite is based apparently upon resistance to macrophage lysosomal enzymic digestion.