Multiplexing of multicolor bioluminescence resonance energy transfer.

  title={Multiplexing of multicolor bioluminescence resonance energy transfer.},
  author={Billy Breton and {\'E}tienne Sauvageau and Joris Zhou and H{\'e}l{\`e}ne Bonin and Christian Le Gouill and Michel Bouvier},
  journal={Biophysical journal},
  volume={99 12},
Bioluminescence resonance energy transfer (BRET) is increasingly being used to monitor protein-protein interactions and cellular events in cells. However, the ability to monitor multiple events simultaneously is limited by the spectral properties of the existing BRET partners. Taking advantage of newly developed Renilla luciferases and blue-shifted fluorescent proteins (FPs), we explored the possibility of creating novel BRET configurations using a single luciferase substrate and distinct FPs… CONTINUE READING
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